Part:BBa_K4359002:Design
Anti-HER2 Affibody- ZHER2:342
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 94
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence is directly adapted from reference [1].
Source
This part is derived from composite part (BBa_K4359006), which was provided by Dr. Vipul Gujrati, TUM Munich, as described in reference [1].
References
[1] Gujrati, V., Kim, S., Kim, S.-H., Min, J. J., Choy, H. E., Kim, S. C., & Jon, S. (2014). Bioengineered Bacterial Outer Membrane Vesicles as Cell-Specific Drug-Delivery Vehicles for Cancer Therapy. ACS Nano, 8(2), 1525–1537. https://doi.org/10.1021/nn405724x
[2] Feldwisch, J., Tolmachev, V., Lendel, C., Herne, N., Sjöberg, A., Larsson, B., Rosik, D., Lindqvist, E., Fant, G., Höidén-Guthenberg, I., Galli, J., Jonasson, P., & Abrahmsén, L. (2010). Design of an Optimized Scaffold for Affibody Molecules. Journal of Molecular Biology, 398(2), 232–247. https://doi.org/10.1016/j.jmb.2010.03.002
[3] Eigenbrot, C., Ultsch, M., Dubnovitsky, A., Abrahmsén, L., & Härd, T. (2010). Structural basis for high-affinity HER2 receptor binding by an engineered protein. Proceedings of the National Academy of Sciences, 107(34), 15039–15044. https://doi.org/10.1073/pnas.1005025107
[4] Nygren, P.-Å. (2008). Alternative binding proteins: Affibody binding proteins developed from a small three-helix bundle scaffold: Affibody binding proteins. FEBS Journal, 275(11), 2668–2676. https://doi.org/10.1111/j.1742-4658.2008.06438.x
[5] Ekerljung, L., Lennartsson, J., & Gedda, L. (2012). The HER2-Binding Affibody Molecule (ZHER2∶342)2 Increases Radiosensitivity in SKBR-3 Cells. PLoS ONE, 7(11), e49579. https://doi.org/10.1371/journal.pone.0049579